Analysis of solute-protein interactions and solute-solute competition by zonal elution affinity chromatography

Analysis of Free Solute Fractions and Solute-Protein Interactions Using Ultrafast Affinity Extraction and Affinity Microcolumns

Effects of Solute-Solute Interactions on Protein Stability Studied Using Various Counterions and Dendrimers

Much work has been performed on understanding the effects of additives on protein thermodynamics and degradation kinetics, in particular addressing the Hofmeister series and other broad empirical phenomena. Little attention, however, has been paid to the effect of additive-additive interactions on proteins. Our group and others have recently shown that such interactions can actually govern prot...

Solute-binding protein-dependent ABC transporters are responsible for solute efflux in addition to solute uptake.

The ATP-binding cassette (ABC) transporter superfamily is one of the most widespread of all gene families and currently has in excess of 1100 members in organisms ranging from the Archaea to manQ1. The movement of the diverse solutes of ABC transporters has been accepted as being strictly unidirectional, with recent models indicating that they are irreversible. However, contrary to this paradig...

Concentration Polarization Phenomena during Dead-end Ultrafiltration of Protein Mixtures. the Influence of Solute-solute Interactions

The flux decline behaviour of some charged proteins and of binary mixtures of charged solutes during unstirred dead-end ultrafiltration has been studied. The mixtures consisted of the proteins bovine serum albumin, (BSA), cy-lactalbumin and/or lysozyme. Of special interest were a-lactalbumin and lysozyme because these proteins are physico-chemically identical, except for the sign of their charg...

Solvation of diclofenac in water from atomistic molecular dynamics simulations - interplay between solute-solute and solute-solvent interactions.

The solubility-permeability relationship of active pharmaceutical ingredients determines the efficacy of their usage. Diclofenac (DCL), which is a widely used nonsteroidal anti-inflammatory drug, is characterized by extremely good membrane permeability, but low water solubility limiting drug effectiveness. The present research focuses on the fundamental explanation of this limitation using the ...